Regulatory

Part:BBa_K4092017

Designed by: Emiliano González Castañón, Juan Antonio Alfaro Almaguer & Emilio Fabian Ortiz   Group: iGEM21_Tec-Monterrey   (2021-10-19)


Toehold for Bois Noir 3

Toehold switches are RNA tools used for the detection of a sequence of interest. They're composed of four main parts: the first part is located at the beginning of the toehold and it is complementary to a given sequence of interest also called trigger; next, there is a hairpin-like sequence, which hides an RBS and start codon; subsequently, at the end of the hairpin, a linker sequence is used as a spacer before the next component; finally, a coding sequence for a reporter gene of choice is added after the toehold design (not present in this part). This toehold is functional at a temperature of 37°C for the detection of Candidatus phytoplasma solani (PlsC gene). It has a MFE of -21.2999992370606 (kcal/mol) and was designed using the software Toehold Switch Creator by the Tec-Monterrey 2021 team.



Usage and Biology

In order to have a more graphical approach of the comparison of the structures, we obtained an image of the secondary structure of the toeholds using the NUPACK package.

Fig. Toehold secondary structure at 37°C.
Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None